PhytophthoraV1, Main, Exploration, bibRecord, 000996

Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco.

Identifieur interne : 000996 ( Main/Exploration ); précédent : 000995; suivant : 000997

Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco.

Auteurs : Claire Rosnoblet [France] ; Hervé Bègue [France] ; Cécile Blanchard [France] ; Carole Pichereaux [France] ; Angélique Besson-Bard [France] ; Sébastien Aimé [France] ; David Wendehenne [France]

Source :

Plant, cell & environment [ 1365-3040 ] ; 2017.

RBID : pubmed:26662183

Descripteurs français

KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Cellules végétales (effets des médicaments et des substances chimiques), Cellules végétales (métabolisme), Chromatographie sur gel (MeSH), Liaison aux protéines (effets des médicaments et des substances chimiques), Mort cellulaire (effets des médicaments et des substances chimiques), Nitrosation (MeSH), Protéine contenant la valosine (métabolisme), Protéines fongiques (pharmacologie), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Tabac (génétique), Tabac (immunologie), Tabac (métabolisme).
MESH :
- effets des médicaments et des substances chimiques : Cellules végétales, Liaison aux protéines, Mort cellulaire, Régulation de l'expression des gènes végétaux.
- génétique : ARN messager, Tabac.
- immunologie : Tabac.
- métabolisme : ARN messager, Cellules végétales, Protéine contenant la valosine, Protéines végétales, Tabac.
- pharmacologie : Protéines fongiques.
- Chromatographie sur gel, Nitrosation.

English descriptors

KwdEn :
- Cell Death (drug effects), Chromatography, Gel (MeSH), Fungal Proteins (pharmacology), Gene Expression Regulation, Plant (drug effects), Nitrosation (MeSH), Plant Cells (drug effects), Plant Cells (metabolism), Plant Proteins (metabolism), Protein Binding (drug effects), RNA, Messenger (genetics), RNA, Messenger (metabolism), Tobacco (genetics), Tobacco (immunology), Tobacco (metabolism), Valosin Containing Protein (metabolism).
MESH :
- chemical , genetics : RNA, Messenger.
- chemical , metabolism : Plant Proteins, RNA, Messenger, Valosin Containing Protein.
- chemical , pharmacology : Fungal Proteins.
- drug effects : Cell Death, Gene Expression Regulation, Plant, Plant Cells, Protein Binding.
- genetics : Tobacco.
- immunology : Tobacco.
- metabolism : Plant Cells, Tobacco.
- Chromatography, Gel, Nitrosation.

Abstract

Cdc48, a molecular chaperone conserved in different kingdoms, is a member of the AAA+ family contributing to numerous processes in mammals including proteins quality control and degradation, vesicular trafficking, autophagy and immunity. The functions of Cdc48 plant orthologues are less understood. We previously reported that Cdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. Here, we inv estigated the function of NtCdc48 in cryptogein signalling and induced hypersensitive-like cell death. NtCdc48 was found to accumulate in elicited cells at both the protein and transcript levels. Interestingly, only a small proportion of the overall NtCdc48 population appeared to be S-nitrosylated. Using gel filtration in native conditions, we confirmed that NtCdc48 was present in its hexameric active form. An immunoprecipitation-based strategy following my mass spectrometry analysis led to the identification of about a hundred NtCdc48 partners and underlined its contribution in cellular processes including targeting of ubiquitylated proteins for proteasome-dependent degradation, subcellular trafficking and redox regulation. Finally, the analysis of cryptogein-induced events in NtCdc48-overexpressing cells highlighted a correlation between NtCdc48 expression and hypersensitive cell death. Altogether, this study identified NtCdc48 as a component of cryptogein signalling and plant immunity.

DOI: 10.1111/pce.12686
PubMed: 26662183

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000D12
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Le document en format XML

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<front><div type="abstract" xml:lang="en">Cdc48, a molecular chaperone conserved in different kingdoms, is a member of the AAA+ family contributing to numerous processes in mammals including proteins quality control and degradation, vesicular trafficking, autophagy and immunity. The functions of Cdc48 plant orthologues are less understood. We previously reported that Cdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. Here, we inv estigated the function of NtCdc48 in cryptogein signalling and induced hypersensitive-like cell death. NtCdc48 was found to accumulate in elicited cells at both the protein and transcript levels. Interestingly, only a small proportion of the overall NtCdc48 population appeared to be S-nitrosylated. Using gel filtration in native conditions, we confirmed that NtCdc48 was present in its hexameric active form. An immunoprecipitation-based strategy following my mass spectrometry analysis led to the identification of about a hundred NtCdc48 partners and underlined its contribution in cellular processes including targeting of ubiquitylated proteins for proteasome-dependent degradation, subcellular trafficking and redox regulation. Finally, the analysis of cryptogein-induced events in NtCdc48-overexpressing cells highlighted a correlation between NtCdc48 expression and hypersensitive cell death. Altogether, this study identified NtCdc48 as a component of cryptogein signalling and plant immunity.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26662183</PMID>
<DateCompleted><Year>2017</Year>
<Month>12</Month>
<Day>04</Day>
</DateCompleted>
<DateRevised><Year>2017</Year>
<Month>12</Month>
<Day>04</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3040</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>40</Volume>
<Issue>4</Issue>
<PubDate><Year>2017</Year>
<Month>Apr</Month>
</PubDate>
</JournalIssue>
<Title>Plant, cell & environment</Title>
<ISOAbbreviation>Plant Cell Environ</ISOAbbreviation>
</Journal>
<ArticleTitle>Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco.</ArticleTitle>
<Pagination><MedlinePgn>491-508</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1111/pce.12686</ELocationID>
<Abstract><AbstractText>Cdc48, a molecular chaperone conserved in different kingdoms, is a member of the AAA+ family contributing to numerous processes in mammals including proteins quality control and degradation, vesicular trafficking, autophagy and immunity. The functions of Cdc48 plant orthologues are less understood. We previously reported that Cdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. Here, we inv estigated the function of NtCdc48 in cryptogein signalling and induced hypersensitive-like cell death. NtCdc48 was found to accumulate in elicited cells at both the protein and transcript levels. Interestingly, only a small proportion of the overall NtCdc48 population appeared to be S-nitrosylated. Using gel filtration in native conditions, we confirmed that NtCdc48 was present in its hexameric active form. An immunoprecipitation-based strategy following my mass spectrometry analysis led to the identification of about a hundred NtCdc48 partners and underlined its contribution in cellular processes including targeting of ubiquitylated proteins for proteasome-dependent degradation, subcellular trafficking and redox regulation. Finally, the analysis of cryptogein-induced events in NtCdc48-overexpressing cells highlighted a correlation between NtCdc48 expression and hypersensitive cell death. Altogether, this study identified NtCdc48 as a component of cryptogein signalling and plant immunity.</AbstractText>
<CopyrightInformation>© 2015 John Wiley & Sons Ltd.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rosnoblet</LastName>
<ForeName>Claire</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, Université de Bourgogne Franche-Comté, UMR 1347 Agroécologie, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bègue</LastName>
<ForeName>Hervé</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, Université de Bourgogne Franche-Comté, UMR 1347 Agroécologie, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Blanchard</LastName>
<ForeName>Cécile</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, Université de Bourgogne Franche-Comté, UMR 1347 Agroécologie, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pichereaux</LastName>
<ForeName>Carole</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Fédération de Recherche 3450, Agrobiosciences, Interactions et Biodiversité, CNRS, 31326, Castanet-Tolosan, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institut de Pharmacologie et de Biologie Structurale - CNRS, Université de Toulouse, 205 route de Narbonne,, 31077, Toulouse, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Besson-Bard</LastName>
<ForeName>Angélique</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, Université de Bourgogne Franche-Comté, UMR 1347 Agroécologie, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Aimé</LastName>
<ForeName>Sébastien</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>INRA, UMR 1347 Agroécologie, Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wendehenne</LastName>
<ForeName>David</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Pôle Mécanisme et Gestion des Interactions Plantes-Microorganismes - ERL CNRS 6300, Université de Bourgogne Franche-Comté, UMR 1347 Agroécologie, 17 rue Sully, BP 86510, 21065, Dijon cédex, France.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>01</Month>
<Day>18</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Plant Cell Environ</MedlineTA>
<NlmUniqueID>9309004</NlmUniqueID>
<ISSNLinking>0140-7791</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C061032">cryptogein protein, Phytophthora cryptogea</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.6.4.6</RegistryNumber>
<NameOfSubstance UI="D000074405">Valosin Containing Protein</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002850" MajorTopicYN="N">Chromatography, Gel</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015538" MajorTopicYN="N">Nitrosation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000074405" MajorTopicYN="N">Valosin Containing Protein</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cdc48 partners</Keyword>
<Keyword MajorTopicYN="N">NtCdc48</Keyword>
<Keyword MajorTopicYN="N">cell death</Keyword>
<Keyword MajorTopicYN="N">cryptogein</Keyword>
<Keyword MajorTopicYN="N">defence responses</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year>
<Month>09</Month>
<Day>02</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2015</Year>
<Month>11</Month>
<Day>20</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2015</Year>
<Month>11</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2015</Year>
<Month>12</Month>
<Day>15</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>12</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2015</Year>
<Month>12</Month>
<Day>15</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">26662183</ArticleId>
<ArticleId IdType="doi">10.1111/pce.12686</ArticleId>
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<affiliations><list><country><li>France</li>
</country>
<region><li>Bourgogne</li>
<li>Bourgogne-Franche-Comté</li>
<li>Midi-Pyrénées</li>
<li>Occitanie (région administrative)</li>
</region>
<settlement><li>Castanet-Tolosan</li>
<li>Dijon cédex</li>
<li>Toulouse</li>
</settlement>
</list>
<tree><country name="France"><region name="Bourgogne-Franche-Comté"><name sortKey="Rosnoblet, Claire" sort="Rosnoblet, Claire" uniqKey="Rosnoblet C" first="Claire" last="Rosnoblet">Claire Rosnoblet</name>
</region>
<name sortKey="Aime, Sebastien" sort="Aime, Sebastien" uniqKey="Aime S" first="Sébastien" last="Aimé">Sébastien Aimé</name>
<name sortKey="Begue, Herve" sort="Begue, Herve" uniqKey="Begue H" first="Hervé" last="Bègue">Hervé Bègue</name>
<name sortKey="Besson Bard, Angelique" sort="Besson Bard, Angelique" uniqKey="Besson Bard A" first="Angélique" last="Besson-Bard">Angélique Besson-Bard</name>
<name sortKey="Blanchard, Cecile" sort="Blanchard, Cecile" uniqKey="Blanchard C" first="Cécile" last="Blanchard">Cécile Blanchard</name>
<name sortKey="Pichereaux, Carole" sort="Pichereaux, Carole" uniqKey="Pichereaux C" first="Carole" last="Pichereaux">Carole Pichereaux</name>
<name sortKey="Pichereaux, Carole" sort="Pichereaux, Carole" uniqKey="Pichereaux C" first="Carole" last="Pichereaux">Carole Pichereaux</name>
<name sortKey="Wendehenne, David" sort="Wendehenne, David" uniqKey="Wendehenne D" first="David" last="Wendehenne">David Wendehenne</name>
</country>
</tree>
</affiliations>
</record>

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Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco.

Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco.

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